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Biosynthesis of zinc oxide nanoparticles using Stephania abyssinica leaves aqueous extract and evaluation of its antibacterial activities
Zinc oxide nanoparticles (ZnO NPs) are used in a variety of applications. ZnO NPs were synthesized from 90 mL of Stephania abyssinica leaf extract with 60 mL of 0.1M Zn (NO3)2.6H2O precursor in a 60 mL of 0.5M NaOH. UV-Visible spectroscopy, FTIR, and XRD techniques were used to characterize the synthesized ZnO NPs. Phytochemicals were examined using a standard qualitative test in the Stephania abyssinica aqueous leaves extract. A disc diffusion technique was employed to assess ZnO NPs' antibacterial efficacy. The result indicated that a change of color from colorless to pale to yellow due to the formation of ZnO NPs. The UV-Vis at 372 nm and the FT-IR at 433 cm–1 indicated the formation of a Zn-O bond. The inhibition zone of ZnO NPs, plant extract, and gentamycin were 22 ± 0.357, 12 ± 0.451, and 25 ± 0.317 mm respectively for Gram-positive (S. aureus) bacteria and Gram-negative bacteria by the ZnO NPs.
