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Synthesis of Zinc oxide nanoparticles using Rhamnus prinoides leaves aqueous extract and evaluation of its antibacterial activities
Zinc oxide nanoparticles (ZnO) are used in a variety of applications. ZnO NPs were synthesized from 90 mL of Rhamnus prinoides leaf extract with 60 mL of 0.1M Zn (NO3)2.6H2O precursor in a 60 mL of 0.5M NaOH. Uv-visible spectroscopy, FTIR, and XRD techniques were used to characterize the synthesized ZnO NPs. Phytochemicals were examined using a standard qualitative test in the Rhamnus prinoides aqueous leaves extract. A disc diffusion technique was employed to assess ZnO NPs' antibacterial efficacy. The result indicated a change from colorless to pale yellow due to the formation of ZnO NPs. The Uv-Vis at 372 nm and the FT-IR at 433 cm–1 indicated the formation of a Zn-O bond. The inhibition zone of ZnO NPs, plant extract, and gentamycin were 22 ± 0.357, 12 ± 0.451, and 25 ± 0.317 mm respectively for gram-positive (S. aureus) bacteria. The diameter inhibition zone of the synthesized ZnO NPs, plant extract, and gentamycin were 22 ± 0.357 mm, 12 ± 0.451 mm, and 25 ± 0.317mm, respectively for gram-positive (S. aureus) bacteria. The corresponding inhibition zone with gram-negative (E. coli) bacteria was 20 ± 0.102 mm, 11 ± 0.471 mm, and 24 ± 0.254 mm, respectively.
